《植物生理学报》 2015, 51(10): 1625-1632

水稻磷脂酶Dζ2在响应磷饥饿胁迫过程中的作用

姚腾, 孙林啸, 吕玮鑫, 洪月云^*

华中农业大学作物遗传改良国家重点实验室, 武汉430070

通信作者：洪月云；E-mail: hongyy@mail.hzau.edu.cn；Tel: 027-87280545

摘　要：

通过分子遗传突变分析水稻(Oryza sativa)磷脂酶Dζ2 (PLDζ2)在应答磷饥饿过程中的作用, 结果表明水稻PLDζ2被磷饥饿胁迫显著诱导, 其缺失突变导致植株在低磷条件下生长受阻, 根生长显著低于野生型; 在严重缺磷条件下, 突变体pldζ2-1根长仅为野生型的58%, 其根系体积仅为野生型的40%, 突变体植株地上部生长也明显受到抑制。在磷饥饿胁迫条件下, 突变体pldζ2-1体内PLDζ2催化磷脂酰胆碱(PC)产生磷脂酸(PA)的活性明显低于野生型, 并使糖脂合成相关基因如磷脂酸磷酸水解酶1 (PAH1)和单半乳糖二酰甘油合酶2 (MGD2)的基因表达量显著下调, 表明水稻PLDζ2在磷饥饿胁迫下对根生长具有正调控作用。

关键词：磷脂酶Dζ2; 磷饥饿; 脂质代谢; 植物生长

收稿：2015-07-28   修定：2015-09-11

资助：国家自然科学基金(30971852)。

The Role of Phospholipase Dζ2 in Response to Phosphate Starvation in Rice

YAO Teng, SUN Lin-Xiao, LÜ Wei-Xin, HONG Yue-Yun^*

National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China

Corresponding author: HONG Yue-Yun; E-mail: hongyy@mail.hzau.edu.cn; Tel: 027-87280545

Abstract:

In this study, the role of phospholipase Dζ2 (PLDζ2) in response to phosphate starvation was analyzed through genetic mutation of PLDζ2 gene in rice (Oryza sativa). The result shows that the transcript level of rice PLDζ2 was largely induced by phosphate starvation. Loss of PLDζ2 resulted in growth inhibition; the root length of the pldζ2-1 mutant was 58% of wild type (WT), and root volume of the pldζ2-1 mutant was 40% of WT under phosphate starvation condition. PLDζ2 activity of the pldζ2-1 mutant was less than that of WT when plants were grown under phosphate starvation condition. The transcript levels of genes, such as phosphatidic acid phosphohydrolase 1 (PAH1) and monogalactosyldiacylglycerol synthase 2 (MGD2) genes involved in galactolipid synthesis, were significantly reduced in the pldζ2-1 mutant compared with WT in response to phosphate starvation. The results suggest that rice PLDζ2 plays a positive role in growth regulation under phosphate starvation condition.

Key words: phospholipase Dζ2; phosphate starvation; lipid metabolism; plant growth